ydaK [2018-02-11 19:03:31]

general stress protein, may act as c-di-GMP receptor, required for extracellular polysaccharide synthesis by YdaL-YdaM-YdaN

locus: BSU04280

pI: 6.70

mw: 32.53 kDa

protein length: 283 aa

gene length: 849 bp

function: unknown

product: general stress protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.2|Targets of c-di-GMP]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 480,013 → 480,864

The protein

[SW|Domains]

• four transmembrane helices at the N-terminus, they are essential fro activation of EPS formation by [protein|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|YdaL], [protein|961538BEDD27C24FC073AFB7AB3268E3D46783CC|YdaM], and [protein|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|YdaN] [pubmed|28536559]
• contains a C-terminal degenerated [SW|GGDEF domain] [Pubmed|23893111]

[SW|Cofactors]

• binds c-di-GPM at high concentration (K(D) for c-di-GMP: 1.1 myM) [Pubmed|23893111]

[SW|Localization]

• cell membrane, forms clusters at the cell poles and septa (with [protein|961538BEDD27C24FC073AFB7AB3268E3D46783CC|YdaM] and [protein|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|YdaN]) [Pubmed|27897378]

Expression and Regulation

Operons

• genes: [gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|22383849], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • expressed during stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C074 (ydaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2072 NBRP B. subtilis, Japan]
• GP1592 (kan) available in [SW|Jörg Stülke]'s lab
• JH642 and its derivatives: natural deletion of the 18 kb [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|353DADE8E57A0F1895CCEB62701F9273EEB5EB45|mntH] region encompassing the genes [gene|467E9CF20B545A976614968E7DD5497F9390D344|ydzA]-[gene|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB]-[gene|69DEE29383E40F49890CC8314DFBF56D70D35113|ydaJ]-[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]-[gene|28D80C5497AE29A24AD6B9A1A713A2B3915F2CED|ydaL]-[gene|961538BEDD27C24FC073AFB7AB3268E3D46783CC|ydaM]-[gene|ADE0122D8FFF2F78B6D21031B7D02A323A13ED63|ydaN]-[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]-[gene|6085BDAD40F719555EA4EEA7BBAD74103BD03D0F|mutT]-[gene|2883FC60CD69948D5C43BDDFEFBE7A1CA7C743C7|ydaP]-[gene|87834D5C47064BEFF26F086A8202C311F28F9D38|ydzK]-[gene|search|mntH ][pubmed|18670626]
• BKE04280 (Δ[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAAATAAGCAAATAATTTTT, downstream forward: _UP4_ATGAATGAACTATAAAGGAA
• BKK04280 (Δ[gene|70ED3CE683F3A3F785480F40986CABE7729C17C7|ydaK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAAATAAGCAAATAATTTTT, downstream forward: _UP4_ATGAATGAACTATAAAGGAA

References

Reviews

• 28296273

Original publications

• 22383849,22821967,23893111,27897378,28536559